Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) Monoclonal Antibody (ENO2, 2706)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Tested in direct ELISA (Use antibody at 2–4 µg/mL for coating).
• For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme involved in glycolysis and gluconeogenesis, catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate.
NSE is highly stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid upon neuronal injury.
The gamma-subunit (ENO2) is primarily expressed in neurons and neuroendocrine cells.
Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms.
NSE antibodies are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells.
Release of NSE from damaged neurons serves as a biomarker for neuronal injury and is clinically used as a marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis.
Elevated NSE levels are also observed in neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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