Thermo Fisher Scientific Fractalkine Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Immunogen

Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the specified buffer. This antibody is specific for human Fractalkine protein. BLAST analysis suggests 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with Fractalkine from other sources has not been determined.

Target Information

Chemokines are a family of proteins involved in leukocyte trafficking during immune surveillance and inflammatory recruitment. CX3CL1 (Fractalkine) is a CX3C chemokine that mediates adhesion and migration of leukocytes via membrane-bound and soluble forms. It can suppress production of nitric oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting a role as an intrinsic inhibitor against neurotoxicity. Its receptor, CX3CR1, also serves as a co-receptor for HIV-1 and HIV-2 envelope fusion and infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.