Thermo Fisher Scientific CD63 Monoclonal Antibody (H5C6), eFluor 450, eBioscience

Thermo Fisher Scientific CD63 Monoclonal Antibody (H5C6), eFluor 450, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1 µg)/test
Publications: View 10 publications

Product Specifications

Additional Formats:

• eFluor 660
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. Cell surface expression of CD63 can be detected on activated basophils, platelets, monocytes, macrophages, and granulocytes, as well as on endothelial cells, fibroblasts, and smooth muscle cells. CD63 associates with integrins (VLA-3, VLA-6) and TIMP-1 to mediate allergic responses.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested with normal human peripheral blood cells at 5 µL (1 µg) per test.
A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. Emits at 445 nm and is excited with the Violet laser (405 nm). Ensure instrument compatibility.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD63 (LAMP-3, lysosome-associated membrane protein-3) is a glycoprotein of the tetraspanin family found in late endosomes, lysosomes, and secretory vesicles. It is also present on the plasma membrane following cell activation and serves as a basophil activation marker. CD63 interacts with integrins, affects phagocytosis and migration, and is involved in trafficking regulation of ROMK1 channels. It functions as a T-cell costimulation molecule and is used as a prognostic marker in early-stage carcinomas. CD63 is expressed on activated platelets, monocytes, macrophages, and weakly on granulocytes, B, and T cells. It is identical to melanoma-associated antigen ME491 and platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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