Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eFluor 660, eBioscience
Mouse 및 Human CD11b 검출용 M1/70 단클론 항체로, eFluor 660 형광 표지. Flow Cytometry 및 IHC(Frozen)에 적합하며, Mac-1 복합체 인식. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 43 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 4 |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 2 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 19 |
| Flow Cytometry (Flow) | 0.06 µg/test | 242 |
| Immunoprecipitation (IP) | - | 1 |
| Neutralization (Neu) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_11218677 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395~805, Brilliant Violet 421~786, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue/Yellow series, PE, PE-Cyanine5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436~780, Custom conjugation available.
Product Specific Information
Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form Mac-1 (alphaM-beta2 integrin) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneum. Cross-reactive with human CD11b, allowing detection in human monocytes, granulocytes, and NK cell subsets. CD11b participates in adhesive cell interactions.
Applications Reported:
Flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested:
- Flow cytometry: ≤ 0.06 µg/test (100 µL final volume).
- IHC (Frozen): ≤ 5 µg/mL.
Not recommended for IHC of formalin-fixed paraffin-embedded tissue.
Optimize dilution for best performance.
Fluorophore Information:
eFluor® 660 is an alternative to Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM) forms a complex with CD18 (integrin beta-2) to create Mac-1 or CR3. Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. Plays key roles in cell adhesion and immune response regulation. CD11b expression varies by cell type and tissue, increasing during monocyte maturation. Associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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