Thermo Fisher Scientific TFAM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2.5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

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Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide region of human TFAM.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2255882

Product Specific Information

Recommended positive controls: bEND.3, Hep3B, HepG2, Jurkat, K562.

Predicted reactivity: Cow: 92%; Dog: 83%; Guinea Pig: 75%; Horse: 92%; Mouse: 75%; Pig: 92%; Rabbit: 83%.

By Western blot, PA1-24435 detects the ~ 29 kDa TFAM protein.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.