Thermo Fisher Scientific Transferrin Receptor Monoclonal Antibody (DF1513)
인간 CD71(Transferrin Receptor)을 인식하는 Mouse IgG1 단일클론 항체. ICC/IF 및 Flow Cytometry에 적합하며, 1 mg/mL 농도의 액상 형태로 제공. 단백질 A 정제, PBS 버퍼 및 0.09% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:250 |
| Flow Cytometry (Flow) | 1:10–1:25 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mustelid, Rhesus monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DF1513 |
| Immunogen | KG1 cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538138 |
Additional Formats
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Human CD71 antibody, clone DF1513, recognizes the human CD71 cell surface antigen, an approximately 190 kDa homodimeric glycoprotein expressed by proliferating cells.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor.
Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from conditions such as rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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