Thermo Fisher Scientific Transferrin Receptor Monoclonal Antibody (10F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:40

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

1:2

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

10F11

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10966364

Product Specific Information

MA5-11441 reacts with human and mouse samples in IHC (P), FACS and Western blot applications. This antibody is not suitable for MCF-7 or NIH-3T3 cells in Western blot analysis.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.