Thermo Fisher Scientific Phospho-NPM1 (Ser10) Polyclonal Antibody
Phospho-NPM1 (Ser10)에 특이적인 Rabbit Polyclonal Antibody로, 인산화된 Nucleophosmin 검출에 적합합니다. Western blot, IHC, ICC 등 다양한 응용 가능. 고순도 정제 및 안정적 PBS/glycerol buffer 보존. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NPM1 (Accession P06748), corresponding to amino acid residues around phosphorylated Ser10. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816901 |
Product Specific Information
Antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Ser10.
Target Information
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in:
- Ribosome assembly and transport
- Cytoplasmic/nuclear trafficking
- Regulation of DNA polymerase alpha activity
- Centrosome duplication
- Regulation of p53
NPM1 shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones. It binds nucleic acids, prevents protein aggregation, protects enzymes during thermal denaturation, and assists renaturation.
NPM1 is associated with centrosomes and acts as a substrate for CDK2/cyclin E during duplication.
It interacts with tumor-associated fusion proteins (NPM-ALK, NPM-RAR, NPM-MLF1) and may facilitate oncogenic conversion.
Additionally, NPM1 sequesters tumor suppressor RF in the nucleolus, protecting it until required.
Dysfunction of NPM1 is linked to diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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