Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), NovaFluor Yellow 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.1 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436–780
• NovaFluor Blue 610–120S, Red 710, Yellow 610
• Custom conjugation available upon request

Product Specific Information

Description:
The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8α/β heterodimer or CD8α/α homodimer. It is present on most thymocytes and a subset of mature T cells and NK cells. CD8 binds to MHC class I and, through p56lck association, contributes to T-cell development and activation.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis.

Applications Tested:
Pre-titrated and tested for flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures that enable engineered fluorescent signatures with minimized spillover and spread.

Excitation / Emission / Laser:
552 nm / 663 nm / 561 nm (Yellow laser)

Target Information

CD8 (Cluster of Differentiation 8):
A type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction, T-cell differentiation, and activation. CD8 is mainly expressed on T cells as a CD8α/β heterodimer and acts as a co-receptor with TCR for MHC class I molecules. CD8α is essential for MHC-I binding and is also found as CD8α/α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.
Upon MHC-I/peptide complex ligation, CD8 recruits Lck kinase, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are key in pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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