Thermo Fisher Scientific Metadherin Monoclonal Antibody (CL0397)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CL0397

Immunogen

Recombinant Protein corresonding to Human Metadherin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Metadherin,uniProtId:Q86UE4-1,ncbiNodeId:9606,antigenRange:315-461,antigenLength:582,antigenImageFileName:MA5-34982_Metadherin_Q86UE4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-34982_Metadherin_Q86UE4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2848887

Product Specific Information

Immunogen sequence: RKTEPSAWSQ DTGDANTNGK DWGRSWSDRS IFSGIGSTAE PVSQSTTSDY QWDVSRNQPY IDDEWSGLNG LSSADPNSDW NAPAEEWGNW VDEERASLLK SQEPIPDDQK VSDDDKEKGE GALPTGKSKK KKKKKKKQGE DNSTAQD

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.