Thermo Fisher Scientific Caldesmon, HMW (h-Caldesmon) Recombinant Mouse Monoclonal Antibody (rCALD1, 820)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

0.25-0.5 µg/mL

-

Peptide Array (Array)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

rCALD1, 820

Immunogen

Recombinant full-length human CALD1 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caldesmon,uniProtId:Q05682-1,ncbiNodeId:9606,antigenRange:1-793,antigenLength:793,antigenImageFileName:800-MSM6-P0_Caldesmon_Q05682-1_House_mouse.svg,antigenImageFileNamePDP:800-MSM6-P0_Caldesmon_Q05682-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

This antibody does not react with rat.

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Target Information

Caldesmon is a developmentally regulated protein involved in smooth muscle and non muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their electrophorectic mobility and cellular distribution. The h caldesmon variant (120 - 150 kDa) is predominantly expressed in smooth muscle whereas l caldesmon (70 - 80 kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.