Thermo Fisher Scientific MGMT Recombinant Rabbit Monoclonal Antibody (JJ089-6)
Recombinant rabbit monoclonal antibody targeting human MGMT protein. Validated for WB, IHC, and ICC/IF applications. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for DNA repair and chemoresistance research.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ089-6 |
| Immunogen | Recombinant protein within Human MGMT aa 1–140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809738 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
O6-Methylguanine-DNA Methyltransferase (MGMT) is a DNA repair protein critical in tumor cell resistance to chemotherapeutic alkylating agents. It protects normal cells against genotoxic and carcinogenic effects of DNA alkylation by removing alkyl groups from the O6 position of guanine. MGMT expression varies by cell type, tissue, species, and tumor characteristics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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