Thermo Fisher Scientific HBEGF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Neutralization (Neu)

0.1-0.6 µg/mL

-

Product Specifications

Published species

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sf 21-derived recombinant human HB-EGF Asp63-Leu148 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HBEGF,uniProtId:Q99075-1,ncbiNodeId:9606,antigenRange:63-148,antigenLength:208,antigenImageFileName:PA5-47352_HBEGF_Q99075-1_Goat.svg,antigenImageFileNamePDP:PA5-47352_HBEGF_Q99075-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605881

Product Specific Information

In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

HBEGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function. HBEGF promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. It also acts as a diphtheria toxin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.