Thermo Fisher Scientific PDHA1 Monoclonal Antibody (9H9AF5)
PDHA1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫드, 소 시료에 반응하며, 미토콘드리아 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.0 µg/mL | View 15 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View publications |
| Flow Cytometry (Flow) | 1 µg/mL | View 1 publication |
| Miscellaneous (PubMed) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9H9AF5 |
| Immunogen | Human recombinant full-length PDH E1-alpha (amino acids 1–390) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_10375326 |
Product Specific Information
For positive control, use isolated mitochondria from Human, Bovine, Rat, and Mouse hearts, HepG2 lysate; cultured normal Human embryonic lung fibroblasts (strain MRC5); HL60 cells.
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle.
The PDH complex includes three enzymatic components:
- Pyruvate dehydrogenase (E1)
- Dihydrolipoamide acetyltransferase (E2)
- Lipoamide dehydrogenase (E3)
The E1 enzyme is a heterotetramer consisting of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit, which contains the active site essential for PDH complex function.
Mutations in PDHA1 are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternatively spliced transcript variants encoding different isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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