Thermo Fisher Scientific ATP5A1 Monoclonal Antibody (7H10BD4F9)
ATP5A1 단클론 항체로 인간, 생쥐, 소, 랫드 시료에서 반응. Western blot, ICC/IF, Flow cytometry 등 다양한 응용 가능. 미토콘드리아 ATP 합성효소 α 서브유닛을 표적. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 38 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 4 publications |
| Flow Cytometry (Flow) | 1 µg/mL | View publications |
| Immunoprecipitation (IP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7H10BD4F9 |
| Immunogen | Bovine Complex V |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2532234 |
Product Specific Information
Use isolated mitochondria from Human, Rat, Bovine, and Mouse heart, and HepG2, cultured human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells for positive controls.
Target Information
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis using an electrochemical proton gradient across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes: the soluble catalytic core (F1) and the membrane-spanning component (Fo), which forms the proton channel.
The catalytic portion comprises five subunits (alpha, beta, gamma, delta, epsilon) with a stoichiometry of 3 alpha, 3 beta, and one each of the others. The proton channel includes subunits a, b, and c.
This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. Pseudogenes are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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