Thermo Fisher Scientific Phospho-Aurora B (Thr232) Polyclonal Antibody
Aurora B 단백질의 Thr232 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능하며, 인간 및 설치류 등 다양한 종에 반응. PBS/glycerol 용액 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AURKB (Accession Q96GD4), corresponding to amino acid residues around phosphorylated Thr232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816499 |
Product Specific Information
This antibody detects endogenous levels of Aurora B (AurB) only when phosphorylated at Threonine 232.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis.
The chromosomal passenger complex (CPC) includes Aurora B along with inner centromere protein (INCENP), survivin, and borealin, which regulate the activity, localization, and stability of Aurora B. Two CPC forms exist during mitosis: a quaternary complex (INCENP, survivin, borealin, AurB) functioning in chromosome alignment and cytokinesis, and a binary INCENP–AurB complex involved in histone H3 modification.
AurB is overexpressed in several tumors, including primary breast and colon cancers, and its expression appears correlated with Aurora-A kinase, suggesting a feedback mechanism between these proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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