Thermo Fisher Scientific Alpha-1-Antitrypsin (SERPINA1) Monoclonal Antibody (AAT, 1379)
인간 Alpha-1-Antitrypsin 단백질을 인식하는 단클론 항체로, Western blot 및 IHC(P)에서 사용 가능. 마우스 IgG1 아이소타입이며 비결합형 액상 형태. 단백질 A/G로 정제되어 안정적이며, 4°C에서 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AAT, 1379 |
| Immunogen | Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (amino acids 41–179 of target protein) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Alpha-1-Antitrypsin (α1-AT) is synthesized in the liver and functions as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which are released during inflammatory reactions in the lung.
Deficiency of α1-AT leads to unregulated protease activity, resulting in pulmonary tissue damage. Alpha-1-AT deficiency is associated with chronic obstructive pulmonary disease (emphysema) and, less commonly, hepatic cirrhosis in infants and neonatal respiratory distress.
Elevated α1-AT levels are observed as an acute phase response in conditions such as rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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