
Thermo Fisher Scientific ERK2 Internal Polyclonal Antibody, DyLight 800
DyLight™ 800 형광 표지된 ERK2 내부 폴리클로날 항체로, Human, Mouse, Rat에 반응합니다. Western blot과 ELISA에 적합하며 높은 특이성과 감도를 제공합니다. MAPK 신호전달 연구용으로 최적화되어 있습니다.
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Thermo Fisher Scientific ERK2 Internal Polyclonal Antibody, DyLight™ 800
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- This DyLight™ 800 conjugated affinity purified antibody is directed against human ERK2 protein.
- Cross-reactivity expected with human, mouse, and rat based on peptide sequence identity.
- Does not react with ERK1 isoform.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase acting as an activator of p90 RSK, MSK, ELK1, and Stat3, involved in the MAPK pathway.
MAP kinases (ERKs) integrate multiple biochemical signals and regulate cellular processes including proliferation, differentiation, transcription regulation, and development.
Activation requires phosphorylation by upstream kinases, and upon activation, ERK2 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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