Thermo Fisher Scientific AMOT Monoclonal Antibody (OTI3F9), TrueMAB
AMOT 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 적용 가능. 인간 시료 반응성, Lyophilized 형태, PBS/trehalose buffer 구성. 연구용으로 angiostatin 관련 혈관 형성 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F9 |
| Immunogen | Full length human recombinant protein of human AMOT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
AMOT (Angiomotin) belongs to the motin family of angiostatin-binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs.
AMOT plays a key role in tight junction maintenance through complexes with ARHGAP17, regulating polarity protein uptake.
It is mainly expressed in endothelial cells of capillaries and larger vessels of the placenta, mediating angiostatin’s inhibitory effect on tube formation and endothelial migration during new blood vessel formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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