Thermo Fisher Scientific HMGB1 Recombinant Rabbit Monoclonal Antibody (SA39-03)
Thermo Fisher Scientific의 HMGB1 재조합 토끼 단클론 항체(SA39-03)는 Western blot, IHC, ICC, Flow cytometry, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 인간, 마우스, 랫트 시료에 반응합니다. 단백질 A로 정제된 액상 항체로, 장기 보관 시 -20°C 보관을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:50,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:1,000 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA39-03 |
| Immunogen | Synthetic peptide within Human HMGB1 aa 151–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809261 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding protein that can act as a proinflammatory cytokine when released extracellularly from damaged or necrotic cells.
Mouse HMGB1 consists of 215 amino acids with three domains:
- Two DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
- C-terminal acidic tail region (30 aa)
Functional regions:
- Nuclear localization signals (NLS): residues 28–44 and 180–185
- Cytokine activity: contained in the B box
- A box: associated with helix-loop-helix transcription factor domains
HMGB1 mediates inflammation, monocyte migration, cytokine secretion, and T cell–dendritic cell interaction. It signals through RAGE, TLR2, and TLR4 receptors. HMGB1 is highly conserved and ubiquitous, serving as a key mediator of sterile and infection-associated inflammation. Elevated HMGB1 levels are observed in tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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