Thermo Fisher Scientific HMGB1 Recombinant Rabbit Monoclonal Antibody (SA39-03)

Applications and Tested Dilution

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Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.

Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding protein that can act as a proinflammatory cytokine when released extracellularly from damaged or necrotic cells.

Mouse HMGB1 consists of 215 amino acids with three domains:

• Two DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
• C-terminal acidic tail region (30 aa)

Functional regions:

• Nuclear localization signals (NLS): residues 28–44 and 180–185
• Cytokine activity: contained in the B box
• A box: associated with helix-loop-helix transcription factor domains

HMGB1 mediates inflammation, monocyte migration, cytokine secretion, and T cell–dendritic cell interaction. It signals through RAGE, TLR2, and TLR4 receptors. HMGB1 is highly conserved and ubiquitous, serving as a key mediator of sterile and infection-associated inflammation. Elevated HMGB1 levels are observed in tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.