Thermo Fisher Scientific Cripto Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지된 Cripto 폴리클로날 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 인간 및 마우스 시료 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the murine cripto protein sequence (between residues 1–50). |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.94 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152986 |
Product Specific Information
In Western blot, a band is seen at approximately 18 kDa representing the core form of Cripto1, and at approximately 36 kDa representing the glycosylated/post-translationally modified form.
Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during early embryo development, and in adults it is reactivated in a wide range of epithelial cancers.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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