Thermo Fisher Scientific Cripto Polyclonal Antibody, DyLight 550
Cripto 단백질을 표적하는 DyLight 550 결합 토끼 폴리클로날 항체로, Western blot, ICC/IF, 유세포분석에 적합합니다. 인간과 생쥐 시료에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 형광 특성은 556/570 nm이며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the murine Cripto protein sequence (between residues 1–50). |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.94 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154481 |
Product Specific Information
In Western blot, a band is seen at approximately 18 kDa representing the core form of Cripto1, and at approximately 36 kDa representing the glycosylated or post-translationally modified form.
Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in embryonic stem (ES) cells and during early embryo development, and is reactivated in various epithelial cancers in adults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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