Thermo Fisher Scientific HCN4 Polyclonal Antibody
Guinea pig 유래 HCN4 폴리클로날 항체로 심장 및 신경 세포의 pacemaker 채널 연구에 적합. WB, IHC, ICC 등 다양한 응용 가능. GST 융합 단백질 항원으로 제작되어 높은 특이성 제공. 동결 건조 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Mouse |
| Host / Isotype | Guinea pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GST fusion protein corresponding to amino acid residues 119–155 of human HCN4 (Intracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2857202 |
Product Specific Information
- Reconstitute antibody with 50 µL of double distilled water (DDW).
- Store reconstituted solution at 4°C for up to 1 week.
- For long-term storage, aliquot and keep at -20°C.
- Avoid repeated freeze-thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g for 5 min).
GST fusion protein sequence:
HGHLHDSAEE RRLIAEGDA SPGEDRTPPG LAAEPERP
Homology:
- Rabbit: identical amino acid residues
- Rat: 35/37 residues identical
- Mouse: 33/37 residues identical
Target Information
HCN4 is a member of the hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channel family.
HCN currents are associated with:
- Pacemaker activity in the heart and brain
- Resting potential control
- Neuronal plasticity
HCN4 channels also function as receptors for sour taste and contribute to pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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