Thermo Fisher Scientific DIABLO Monoclonal Antibody (SMAC 17 1-87)
Smac/DIABLO 단백질을 인식하는 mouse monoclonal antibody로 Western blot, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능. Human, mouse, rat 시료에 반응하며, Protein G 정제된 1 mg/mL 액상 형태로 제공. 세포 사멸 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SMAC 17 1-87 |
| Immunogen | C-Terminal His-tagged recombinant human Smac/DIABLO |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075260 |
Product Specific Information
MA1-936 detects Smac/DIABLO from human, mouse, and rat tissues.
This antibody has been successfully used in Immunoprecipitation, Western blot, Immunohistochemistry (paraffin), Immunofluorescence, and Immunocytochemistry.
By Western blot, it detects a 25-kDa band representing Smac/DIABLO.
The antigen is a C-terminal His-tagged recombinant human Smac/DIABLO.
Target Information
DIABLO (Smac) is a mitochondrial protein that activates various forms of apoptosis, likely through neutralization of one or more members of the IAP (inhibitor of apoptosis) family.
During apoptosis, Smac exits the mitochondria and enters the cytosol.
Mitochondrial-mediated apoptosis is essential for development and tissue homeostasis, and its dysregulation is implicated in malignancies.
Smac/DIABLO also plays a key role in sensitizing cancer cells to apoptosis induced by immune or drug stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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