Thermo Fisher Scientific p300 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-848 detects p300 from human cells.
This antibody has been successfully used in Western blot and ChIP procedures.
By Western blot, PA1-848 detects an approximately 265 kDa protein representing p300 from HeLa cell lysate.

The immunogen is a synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300.
The immunizing peptide (Cat. # PEP-053) is available for use in neutralization and control experiments.

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein.
p300 is related by sequence to CPB (CREB-binding protein), and like CPB, can stimulate transcription through activation of CREB.
This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A.
EP300 also acts as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), playing a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.