Thermo Fisher Scientific MBP Monoclonal Antibody (2H9)
MBP 단백질을 검출하기 위한 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 2H9). Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. 인간, 생쥐, 랫트 반응성. 비결합형 액상 형태로 제공되며 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific MBP Monoclonal Antibody (2H9)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H9 |
| Immunogen | Purified recombinant fragment of human MBP expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11154789 |
Product Specific Information
MA5-15922 targets MBP in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human MBP expressed in E. coli.
MA5-15922 detects MBP, which has a predicted molecular weight of approximately 33 kDa.
Target Information
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also found in bone marrow and the immune system.
These transcripts arise from the long MBP gene (also called Golli-MBP) that contains three additional exons located upstream of the classic MBP exons.
Alternative splicing from the Golli and MBP transcription start sites produces two sets of MBP-related transcripts and gene products:
- Golli mRNAs contain three unique exons spliced in-frame to one or more MBP exons, encoding hybrid proteins with Golli and MBP sequences.
- Classic MBP transcripts contain only MBP exons and produce well-characterized myelin basic proteins.
This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit, important for gene function and regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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