Thermo Fisher Scientific MEK2 N-Term Polyclonal Antibody, HRP
HRP 결합된 MEK2 N-말단 폴리클로날 항체로 인간, 생쥐, 랫트 반응성. Western blot과 ELISA에 사용 가능. 높은 특이성과 민감도를 제공하며 MEK1과 교차 반응하지 않음. 연구용으로 신호전달 경로 분석에 적합.
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Thermo Fisher Scientific MEK2 N-Term Polyclonal Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide near the N-terminus conjugated to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This HRP-conjugated affinity purified antibody is directed against human MEK2 protein.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen.
This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction.
MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222.
Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback loop suppressing MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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