Thermo Fisher Scientific ERK1 C-Term Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL

ELISA

• Tested Dilution: 1:40,000

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This HRP conjugated affinity purified antibody is directed against human ERK1 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that acts as an important activator of p90, RSK, MSK, ELK1, and Stat3, and plays a key role in the MAPK pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
Various stimuli such as growth factors, cytokines, virus infection, and G protein-coupled receptor ligands activate the ERK1 and ERK2 pathways. ERK1 participates in signaling cascades controlling proliferation, differentiation, and cell cycle progression.
Activated ERK1 translocates to the nucleus to phosphorylate nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.