Thermo Fisher Scientific Nestin Polyclonal Antibody
Rabbit polyclonal antibody against human Nestin. Validated for WB and IHC-P applications. Useful for identifying neural stem/progenitor cells and tumor endothelial cells. Supplied as liquid, 0.1 mg/mL in PBS with 40% glycerol. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NES (Nestin), amino acids 1040–1158 (UniProt ID: P48681-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789433 |
Product Specific Information
Immunogen sequence:
DPEGQSQQVG APGLQAPQGL PEAIEPLVED DVAPGGDQAS PEVMLGSEPA MGESAAGAEP GPGQGVGGLG DPGHLTREEV MEPPLEEESL EAKRVQGLEG PRKDLEEAGG LGTEFSELP
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity in tumors.
Highly specific antibodies to human Nestin are useful for characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of neural stem/progenitor cells in CNS disease or injury, and identifying neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic and hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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