Thermo Fisher Scientific SIAH1/SIAH2 Polyclonal Antibody
SIAH1/SIAH2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며 인간, 생쥐, 랫드 반응성. 항원 친화 크로마토그래피로 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 포함.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SIAH1/SIAH2 Polyclonal Antibody
Thermo Fisher Scientific SIAH1/SIAH2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 178–229 of human Siah-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553449 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 40 kDa.
- Purity >95% by SDS-PAGE.
Target Information
SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein expressed in intestinal epithelium and activated during apoptosis.
Human SIAH proteins are produced as two distinct gene products, SIAH-1 and SIAH-2, sharing a highly conserved C-terminal sequence but differing in their N-terminal regions.
SIAH-1 (32 kDa) contains:
- N-terminal RING-finger domain (required for proteolysis)
- Cysteine-rich C-terminal domain (regulates oligomerization and target binding)
As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates its degradation via the ubiquitin-proteasome pathway.
It also binds Bag-1, a Bcl-2 related protein, inhibiting cell growth.
Most SIAH-1 localizes to the nucleus, with a small fraction in the cytoplasm, suggesting interactions with nuclear matrix proteins and DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.