Thermo Fisher Scientific TCR V alpha 3.2 Monoclonal Antibody (RR3-16), FITC, eBioscience

Thermo Fisher Scientific TCR V alpha 3.2 Monoclonal Antibody (RR3-16), FITC

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 5 publications

Product Specifications

Additional Formats

• APC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. TCR specificity is determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, NOD) in the Tcra gene complex.
The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1, DBA/2) haplotypes.
Studies show that the V alpha 3.2 TCR is more highly expressed on CD8+ T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes (≤ 0.125 µg/test).
A test is defined as the amount of antibody that stains a cell sample in 100 µL volume. Recommended titration for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCRs) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response.
TCR recognition of self-peptides is linked to autoimmune diseases, and mutant self-peptides are associated with tumors.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to V beta segments have been found in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially regulating TH1-mediated inflammation.
Most human T cells express TCR alpha-beta and either CD4 or CD8 (single positive). A small subset lacks both (double negative), which increases in autoimmune and immunodeficiency disorders.
Gamma-delta T cells, primarily in epithelia, exhibit less diversity and distinct antigen recognition, with antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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