Thermo Fisher Scientific SERCA1 ATPase Polyclonal Antibody
Rabbit polyclonal antibody against SERCA1 ATPase for WB and ELISA applications. Reacts with human and mouse samples. Supplied as a liquid, affinity-purified, unconjugated antibody. Stored in PBS with glycerol at -20°C. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 901–1001 of human SERCA1/ATP2A1 (NP_775293.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804304 |
Product Specific Information
- Positive Samples: HL-60
- Cellular Location: Endoplasmic reticulum membrane, multi-pass membrane protein, sarcoplasmic reticulum membrane
- Immunogen Sequence:
LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV DPLPMIFKLR ALDLTQWLMV LKISLPVIGL DEILKFVARN YLEDPEDERR K
Target Information
ATP-dependent calcium pumps maintain low cytoplasmic free calcium concentrations. These pumps, located in intracellular organelles, are encoded by the sarcoplasmic/endoplasmic reticulum calcium (SERCA) ATPase family.
- SERCA1: Expressed in type II (fast) skeletal muscle.
- SERCA2: Generates tissue-dependent isoforms: SERCA2a (type I skeletal, cardiac, and smooth muscle) and SERCA2b (ubiquitous expression).
- SERCA3: Found in non-muscle cells, less characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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