Thermo Fisher Scientific ATG5 Polyclonal Antibody, DyLight 650
ATG5 단백질 검출용 DyLight 650 결합 폴리클로날 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 사용 가능. 인간 ATG5 N-말단 합성 펩타이드로 면역화된 Rabbit IgG 항체. 자가포식 및 세포사멸 연구에 적합하며, 4°C 암소 보관.
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Thermo Fisher Scientific ATG5 Polyclonal Antibody, DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to N-terminal region (residues 1–50) of human ATG5 protein |
| Conjugate | DyLight™ 650 |
| Excitation/Emission Max | 651/673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152817 |
Product Specific Information
- In Western Blot, a band is observed at ~56 kDa representing the ATG5-ATG12 complex; molecular weight of human ATG5 is ~33 kDa.
- Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended before immunostaining paraffin tissues.
- Suggested positive control: mouse wildtype ES cell lysate.
Target Information
ATG5 (Autophagy Related 5) is a key component in autophagy and plays roles in apoptosis, mitochondrial quality control, immune response regulation, lymphocyte development, MHC II antigen presentation, and adipocyte differentiation.
Upon conjugation with ATG12, the complex facilitates autophagosome formation and interacts with FADD to contribute to autophagic cell death.
The ATG5-ATG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), modulating interferon production and viral replication.
Dysfunction of ATG5 is linked to spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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