Thermo Fisher Scientific TNF alpha Antibody Cocktail

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 16 publications 16 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Published species

Bacteria, Human

Host/Isotype

Mouse / Ig Mix

Class

Cocktail

Type

Antibody

Clone

68B2B3 and 68B6A3

Immunogen

Purified recombinant human TNF-alpha.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536274

Product Specific Information

This monoclonal mixture is recommended as a capture antibody in Sandwich ELISA applications.

This clone can also be referred to as clone number 68B 2B3 BC6/68B 6A3 1H8 1F2.

Clone 68B2B3 isotype is undetermined. Clone 68B6A3 isotype is IgG1.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.