Thermo Fisher Scientific IFN gamma Monoclonal Antibody (67F12A8), Biotin
인간 IFN-γ 검출용 모노클로날 항체로, Sandwich ELISA에 최적화된 Biotin 결합형 제품입니다. Mouse IgG1, kappa 아이소타입이며 Protein A 정제된 액상 시약입니다. 연구용으로만 사용되며 인간 IFN-γ 특이적으로 반응합니다.
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Applications
ELISA (ELISA)
- Assay-dependent
- View 13 publications
Miscellaneous PubMed (Misc)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 67F12A8 |
| Immunogen | Recombinant human interferon-gamma |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 5 mg/mL BSA |
| Contains | 0.5 mg/mL sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536281 |
Product Specific Information
This product is recommended as a detection antibody in Sandwich ELISA applications.
This clone can also be referred to as clone number 67F 12A8 L1.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits (~45 kDa homodimer). On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFN gamma is species-specific and does not cross-react with mouse.
Functions include:
- Antiviral activity
- Tumor antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
IFN gamma regulates cytokine expression and acts synergistically with other cytokines via the JAK-STAT pathway. It shares ~40% sequence homology with mouse IFN gamma and is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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