Thermo Fisher Scientific GFI1 Monoclonal Antibody (5D7)
Human GFI1 단백질을 검출하는 Mouse IgG1 모노클로날 항체. Western blot과 ELISA에 적합하며, 45kDa 크기의 GFI1 단백질에 특이적으로 반응. 연구용으로만 사용 가능하며 장기 보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific GFI1 Monoclonal Antibody (5D7)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D7 |
| Immunogen | Purified recombinant fragment of human GFI1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10980909 |
Product Specific Information
MA5-15688 targets GFI1 in indirect ELISA and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human GFI1 expressed in E. coli.
MA5-15688 detects GFI1, which has a predicted molecular weight of approximately 45 kDa.
Target Information
GFI1 is a nuclear zinc finger transcriptional repressor involved in hematopoiesis and inner ear development, and is implicated in lymphomagenesis.
Its expression is regulated by the ubiquitin-proteasome system. Ubiquitin ligase Triad1 interacts with the DNA-binding domain of GFI1.
GFI1 represses transcription by binding directly to consensus DNA sequences in target gene promoters.
Deficiency of GFI1 causes neutropenia and accumulation of granulomonocytic precursor cells, resembling myelodysplastic syndrome in mice.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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