Thermo Fisher Scientific GFI1 Monoclonal Antibody (5D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D7

Immunogen

Purified recombinant fragment of human GFI1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10980909

Product Specific Information

MA5-15688 targets GFI1 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15688 immunogen is purified recombinant fragment of human GFI1 expressed in E. Coli.

MA5-15688 detects GFI1 which has a predicted molecular weight of approximately 45kDa.

Target Information

GFI1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis. The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1. Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes. Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.