Thermo Fisher Scientific Ki-67 Monoclonal Antibody (8D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200-1:500

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human, Rat, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8D5

Immunogen

Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH (aa 1100-1113). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ki-67,uniProtId:P46013-1,ncbiNodeId:9606,antigenRange:1100-1113,antigenLength:3256,antigenImageFileName:MA5-15690_Ki-67_P46013-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15690_Ki-67_P46013-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10979995

Product Specific Information

MA5-15690 targets KI67 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.

The MA5-15690 immunogen is synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.

MA5-15690 detects KI67 which has a predicted molecular weight of approximately 358kDa.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.