Thermo Fisher Scientific Ki-67 Monoclonal Antibody (8D5)
Ki-67 단백질을 검출하는 Mouse 모노클로날 항체로, Human 시료에 반응. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 세포 증식 마커 연구에 적합하며, 4°C 또는 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 | View 1 publication |
| ELISA | 1:10,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat, Sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D5 |
| Immunogen | Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67 (aa 1100–1113), conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10979995 |
Product Specific Information
MA5-15690 targets Ki-67 in indirect ELISA, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human Ki-67, conjugated to KLH.
MA5-15690 detects Ki-67 with a predicted molecular weight of approximately 358 kDa.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 expression is cell cycle–dependent, showing perinucleolar localization at G1, nuclear matrix localization at G2, and chromosomal localization during mitosis.
As a proliferation marker, Ki-67 is absent in G0 cells and increases from G1 through mitosis. It is widely used to determine the growth fraction in neoplasms, with a strong correlation between low Ki-67 index and low-grade tumors.
Ki-67 is also utilized as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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