Thermo Fisher Scientific VIM Monoclonal Antibody (OTI13B1), TrueMAB
인간 Vimentin 단백질(AA 210-466)을 면역원으로 한 Mouse Monoclonal Antibody. Western blot에 적합하며, 1:200–1:1,000 희석 사용 권장. 액상 형태로 1 mg/mL 농도, PBS/BSA/glycerol buffer에 보관. -20°C에서 보관하며 동결·해동 반복은 피해야 함.
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Applications
- Western Blot (WB)
Tested Dilution: 1:200–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI13B1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Vimentin, expressed along with desmin during early cellular development, is later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to intermediate filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport into mitochondria and enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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