Thermo Fisher Scientific TNF alpha Polyclonal Antibody, PeproTech
Human TNF-alpha를 인식하는 염소 유래 Polyclonal 항체로, Western blot, ELISA, Neutralization에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로 세포 신호전달 및 염증 관련 연구에 적합.
- 카탈로그번호
- 500-P31AG-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNF alpha Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human TNF-alpha |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930377 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human TNF-alpha. Anti-Human TNF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TNF-alpha matrix.
Sandwich ELISA:
To detect Human TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TNF-alpha (500-P31AGBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TNF-alpha.
Western Blot:
To detect Human TNF-alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TNF-alpha is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell activity, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimeric forms but appears as a 17 kDa band in SDS-PAGE under non-reducing conditions. TNF alpha plays roles in immune responses to infections and tumor necrosis, and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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