Thermo Fisher Scientific CD14 (soluble) Polyclonal Antibody, PeproTech
인간 sCD14 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공합니다. 연구용으로 사용되며, -20°C에서 보관합니다.
- 카탈로그번호
- 500-P320-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD14 (soluble) Polyclonal Antibody, PeproTech
Thermo Fisher Scientific CD14 (soluble) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEK293 cells-derived Recombinant Human sCD14 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930389 |
Product Specific Information
AA Sequence of recombinant protein:
TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human sCD14-specific antibody was purified by affinity chromatography employing an immobilized Human sCD14 matrix.
Sandwich ELISA:
To detect Human sCD14 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sCD14 (500-P320Bt) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human sCD14.
Western Blot:
To detect Human sCD14 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human sCD14 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and is released to serum as soluble and cleaved forms. Soluble sCD14 can recognize structural differences between lipopolysaccharides, contributing to neutralization of serum endotoxins. CD14 also binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same isoform. CD14 dysfunction is associated with mycobacterium chelonae infection and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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