Thermo Fisher Scientific RhoGAP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_0042991) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RhoGAP,uniProtId:Q07960-1,ncbiNodeId:9606,antigenRange:210-439,antigenLength:439,antigenImageFileName:PA5-92051_RhoGAP_Q07960-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92051_RhoGAP_Q07960-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806366

Product Specific Information

Immunogen sequence: VLKYDDFLKS TQKSPATAPK PMPPRPPLPN QQFGVSLQHL QEKNPEQEPI PIVLRETVAY LQAHALTTEG IFRRSANTQV VREVQQKYNM GLPVDFDQYN ELHLPAVILK TFLRELPEPL LTFDLYPHVV GFLNIDESQR VPATLQVLQT LPEENYQVLR FLTAFLVQIS AHSDQNKMTN TNLAVVFGPN LLWAKDAAIT LKAINPINTF TKFLLDHQGE LFPSPDPSGL; Positive Samples: BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung; Cellular Location: Cytoplasm

Target Information

The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.