Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 555, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 555, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor series, FITC, Functional Grade, PE series, PerCP series, Super Bright series, and custom conjugation options available.

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.5 µg/test).
  A test corresponds to staining a cell sample in 100 µL volume; cell number may range from 10⁵–10⁸ cells/test.
  Antibody titration is recommended for optimal performance.

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

Blocking Recommendation:
Each NovaFluor conjugate ships with CellBlox Blocking Buffer.
Add 5 µL per sample when staining with NovaFluor conjugates, including single-color compensation controls.

Optical Properties:

• Excitation: 496 nm
• Emission: 555 nm
• Laser: 488 nm (Blue)

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread. Learn more

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein crucial for T cell signal transduction, differentiation, and activation. It is predominantly expressed on T cells as a CD8αβ heterodimer functioning as a co-receptor for MHC class I molecules. CD8 also exists as CD8αα homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.
Binding of MHC-I/peptide complexes triggers Lck recruitment, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. CTLs are essential for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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