Thermo Fisher Scientific H2BK20ac Polyclonal Antibody
Histone H2BK20ac 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, IP, Dot blot 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 버퍼에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:5,000 |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to human Histone H2BK20ac (acetyl Lys20) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792669 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: HeLa.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Nucleosomes consist of ~146 bp of DNA wrapped around a histone octamer composed of pairs of H2A, H2B, H3, and H4. These structures compact DNA into chromatin, limiting accessibility for transcription, replication, and repair. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability. H2B also exhibits broad antibacterial activity.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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