
Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2/3295)
ACTN2 단백질을 인식하는 mouse monoclonal 항체로, Western blot, IHC 등 다양한 응용에 사용 가능. Human 시료에 반응하며, 단백질 A/G로 정제된 액상 형태. PBS buffer에 0.05% BSA 및 sodium azide 포함, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACTN2/3295 |
| Immunogen | Recombinant fragment (aa 557–692) of human ACTN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Alpha actinins belong to the spectrin gene superfamily, representing a diverse group of cytoskeletal proteins such as alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types.
In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it binds actin to the membrane.
In muscle tissues (skeletal, cardiac, and smooth), isoforms are localized to the Z-disc or dense bodies, anchoring myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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