
Thermo Fisher Scientific Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody (ACTN2, 3294)
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Sarcomeric Actinin Alpha 2(ACTN2)에 특이적인 단클론 항체로, WB, IHC, Peptide Array에 사용 가능. 인간 시료 반응성. 단클론 Mouse IgG2b, kappa 형태로, 단백질 A/G로 정제된 액상 제품. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACTN2, 3294 |
| Immunogen | Recombinant fragment (aa557–692) of human ACTN2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Alpha actinins belong to the spectrin gene superfamily, which includes diverse cytoskeletal proteins such as alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types:
- In nonmuscle cells, it is found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells (skeletal, cardiac, smooth), it is localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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