Thermo Fisher Scientific PBK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:60,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Immunoprecipitation (IP)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center of human PBK. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736328

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS

Predicted Reactivity: Mouse (90%), Rat (93%), Pig (93%), Chimpanzee (99%), Bovine (94%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PBK (TOPK) is a serine/threonine kinase that is related to the dual specific mitogen-activated protein kinase (MAPKK). The kinase is up-regulated in a variety of hematological malignancies, participates in activating lymphoid cells, and supports testicular function (spermatogenesis). Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.