Thermo Fisher Scientific DCP1A Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Control: Target recombinant protein
• Predicted Reactivity: Mouse (94%), Rat (93%), Pig (94%), Bovine (96%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-interacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through an EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF, as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.