Thermo Fisher Scientific JMJD1A Polyclonal Antibody
Human JMJD1A 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 고순도 제품. PBS/glycerol buffer에 보관되며 연구용으로만 사용.
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Thermo Fisher Scientific JMJD1A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KDM3A (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735973 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
JMJD1A is a histone demethylase that specifically demethylates Lys-9 of histone H3, playing a central role in the histone code.
It preferentially acts on mono- and dimethylated H3 Lys-9 residues, with weak or no activity on trimethylated forms.
Demethylation of Lys residues produces formaldehyde and succinate.
JMJD1A is involved in hormone-dependent transcriptional activation via recruitment to androgen-receptor target genes, leading to H3 Lys-9 demethylation and transcriptional activation.
It also plays roles in spermatogenesis by regulating genes such as PRM1 and TMP1, required for sperm chromatin packaging and condensation, and contributes to obesity resistance through metabolic gene regulation (PPARA, UCP1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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