Thermo Fisher Scientific IL-4 Monoclonal Antibody, PeproTech
Human IL-4 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ELISA, 중화 실험에 적합. Protein A로 정제된 Lyophilized 형태이며 PBS buffer에 보관. 연구용으로 면역 조절 관련 연구에 활용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.20–0.50 µg/mL | - |
| ELISA (ELISA) | 0.5–1.0 µg/mL | - |
| Neutralization (Neu) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-4 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929119 |
Product Specific Information
AA Sequence of recombinant protein:
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Preparation:
Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has a broad range of biological actions and shares overlapping functions with IL-13 through the interleukin 4 receptor. STAT6 plays a critical role in mediating IL-4 signaling. The IL-4 gene is located on chromosome 5q within a cytokine gene cluster that includes IL-3, IL-5, IL-13, and CSF2. IL-4 and IL-13 are closely positioned and co-regulated by long-range elements over approximately 120 kb. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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