Thermo Fisher Scientific Cardiac Troponin C Polyclonal Antibody
Cardiac Troponin C 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human TNNC1 (aa 1–161) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2901094 |
Product Specific Information
Positive controls include human heart tissue lysates, MG-63, SKOV-3, SW620, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, and mouse heart tissue.
Target Information
Troponin (Tn) is the central regulatory protein of striated muscle contraction. It consists of three components:
- Tn-I: Inhibitor of actomyosin ATPase
- Tn-T: Contains the binding site for tropomyosin
- Tn-C: Binds calcium ions
The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. This calcium-ion uptake induces allosteric changes in the thin filament, allowing actin-myosin interaction, ATP hydrolysis, and tension generation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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